Mbombela are fourth on the National First Division table and need all three points to join Royal Eagles in third place, which is a playoff spot, while on the other side of the 16-team table, rock-bottom Spurs need every point they can get if they want to survive.
Dlamini says although it will be hard to put the sword into their neighbours, it must be done if they want to realise their dream of qualifying for the promotion playoffs.
"It's a bag of mixed feelings there," Mbombela head coach Milton Dlamini tells snl24.com/kickoff.
"We wouldn't like to see Witbank Spurs go [down], but us on the other side we've got to qualify for the play offs and this is the last chance for us to qualify.
"So ja this game really came at a bad time, the timing is very wrong because everything is at stake. We are looking forward to the game and obviously push to qualify for the playoffs. We wouldn't like to see Spurs go but we have to win, our games are getting finished now, we need to win."
Spurs are fresh from a 5-0 loss to Ajax Cape Town last week, but Dlamini knows that will count for nothing in this game as Spurs players will be looking to impress their new coach Kagisho Dikgacoi.
"You can't read too much into that because it's a derby. You know a derby, it throws away the form book, so they might have conceded five last week but now it's a derby, and the new coach Kagisho Dikgacoi is there. So the players would like to impress the new coach," Dlamini explains.
"So it's gonna be a very, very good encounter. He [Dikgacoi] has played overseas, he has played in the Premier League in England so that is a plus part of it, but on the flip side of it we are not too sure if he knows the Witbank Spurs players enough to change them into a new formation. But I trust him, he has played the game at the highest level, he played in the World Cup.
"There's no chance of a draw, one of the teams will lose, the other one will win. I don't know but the way the stakes are so high there will be no draw tomorrow. The ones that will use their chances on the day will win, but I don't see any of these teams going there for a draw, never, it's not gonna happen."
Kick-off at Puma Stadium is at 15h00.