The ABC Motsepe League side eliminated Ajax Cape Town on penalties in the first round of the competition at the same venue last month after 120 minutes of football produced a 2-2 draw.
Ngubane nearly oversaw another upset in the 2013/14 tournament when Thanda Royal Zulu after squandering a two-goal lead in a thrilling 3-3 stalemate.
"Obviously to me there won't be any stage fright because I've played big games before so there's no stage fright," Ngubane tells snl24.com/kickoff.
"If you remember me at Thanda against Orlando Pirates, who went to three cup finals, so I've been involved in some big games.
"To the players you can never tell. But obviously it's something if we deal with it then that'll give us a better chance to compete. But I'm pulling out all the stops to ensure the boys will be able to handle the game like this.
"It is a situation that is similar to Ajax Cape Town but they were able to deal with it in the correct way but obviously now it's a much bigger stage. If they can deal with the stage fright the same way they did against Ajax Cape Town then I see we got a chance."
Matsatsantsa surprisingly lost this past weekend to relegation-threatened Baroka FC just days after SAFA confirmed that they've been allowed to speak to Stuart Baxter regarding the vacant Bafana Bafana job.
The defeat snapped SuperSport's record 19-game unbeaten run and prevented them from claiming the summit.
Ngubane, who also coached Royal Eagles and African Warriors, says he watched the game with interest.
"Fortunately I watched the game but it may work in two ways: it might work into our advantage or it may put pressure on us," he adds.
"They may come into our game wanting to redeem themselves because, for them to lose that game, regardless they lost under what circumstances, it was also disappointing for them because it may deny them the opportunity to win the league.
"So they may come to this game with some sort of revenge in mind, so it may work in both ways.
"Because, for me when I watched that game, my analysis there wasn't anything that amazed me that was done by Baroka on the day. It was more of SuperSport not being themselves on the day. We can say it is this thing of Stuart Baxter [with SAFA] or they were undermining Baroka because of their position on the log table.
"So if they are able to undermine a team that they are competing with in the same division, how about us as the amateur team? If they can come with that attitude, which is what I'm praying for, to say, 'these are amateurs,' then we may surprise them."
Tickets are still available at Ticket Pro outlets including Jet Stores, Edgars, CNA and Postnet. They are selling at R40 for adults and R15 for the children under the age of 12.
Kick-off time in Clermont is 19h30.