The Cape Town club, who purchased the top-flight status of Mpumalanga Black Aces at the end of last season and relocated to the Mother City, take on their Limpopo visitors at the Cape Town Stadium.
The kick-off time for the game is 19h30, with the club to confirm ticketing details shortly.
City have recently announced a number of new signings, including Shaquille Abrahams (FC Cape Town), Duncan Adonis (Ramblers), Never Ngcuka (Cape United), Ebrahim Seedat (Milano United), Australian Matt Sim (Western Sydney Wanderers, Australia) and Austrian Roland Putsche (Wolfsberger AC).
Coach Eric Tinkler said they expected to announce more names in the coming days.
Cape Town City will also take on Kaizer Chiefs in the quarterfinals of the MTN8 tournament, though the date for that fixture has yet to be announced.