The former Orlando Pirates mentor has been fired after a string of poor results this season that has seen the Team of Choice pick up zero points after four DStv Premiership matches.
Tinkler's side also bowed out of the MTN8 tournament after being beaten by Kaizer Chiefs in the quarter-finals.
According to a source close to Maritzburg, Tinkler met with the club management this morning and both parties agreed to go their separate ways.
"Eric Tinkler is no longer coach at Maritzburg United. The club will make an announcement soon," the source tells KickOff.com.
After saving the club from relegation at the end of the 2018/19 season, Tinkler managed to transfrom the side into a formidable team in the 2019/20 season.
However, ever since the PSL bio-bubble was introduced in August the Pietermaritzburg side have been struggling to pick up positive results.
Tinkler's overall record reads played 57, won 23, drew 13, lost 21 at a rate of 1.44 points per match.
Maritzburg also parted ways with assistant coach Vincent Kobola last month before bringing in Delron Buckley to replace him and Rowen Fernandez as goalkeeper coach.