Sambu took charge of the relegation-threatened outfit at Dan Malesela's expense following the side's 3-2 home loss to Maritzburg United on Tuesday.
While the Chilli Boys are in the Nedbank Cup semi-finals they are just two points above the drop zone with five league matches left.
“It is a big challenge, but a challenge which we must face head on,” Sambu told the club's website ahead of the trip to Soweto.
“We had positive meetings with the senior players as a technical team and everybody at the club leading up to this game.
“The energy in the team has always been a positive one, and it is no different this time around.
“The spirit, hunger and will to fight for the shirt has always been evident, despite good performances, we have not been able to come back with positive results.”
He added: “It is not only about me and the technical team but all of us to unite in the fight to improve our situation in the league.
“We are carrying the hopes of our fans that have given so much to the club.
“The support we have received from the people of the Eastern Cape Province including neutrals, has been tremendous.
“We all know the importance of every game we play, and must go to Orlando with no fear but an intent to go back to Port Elizabeth with a positive result.”